Table 2.
Cell Labeling Conditions | Translational | Post-Translational | |
---|---|---|---|
[35S]Hcy-Protein | [35S]Met-Protein | εN-[35S]Hcy-Lys-Protein | |
% | % | % | |
[35S]Hcy (10 μM, 50 μCi/mL) | 37 | 25 | 38 |
[35S]Hcy + folic acid, 10 μM | <1 | >98 | <1 |
[35S]Hcy + HDL, 1 mg/mL | 68 | 25 | 7 |
[35S]Hcy + Met, 20 μM | 12 | 76 | 12 |
Control, εN-[35S]Hcy-Lys-protein | <4 | 0 | >96 |