TABLE 6.
Reducing the number of rrn operons suppresses both rne-R169Q,ΔCTH and ΔrppH rne-ΔCTH lethalitiesa
| No. of rrn operons deleted (deleted operon[s]) | No. of Lac− colonies/total (ratio) for the indicated mutations |
|
|---|---|---|
| rne-169Q,ΔCTH | ΔrppH rne-ΔCTH | |
| 1 (ΔrrnG) | 0/62 (<0.02) | 0/160 (<0.006) |
| 4 (ΔrrnGADE) | 20/140 (0.14) | 52/252 (0.21) |
| 5 (ΔrrnGADEH) | 16/80 (0.20) | 21/141 (0.12) |
a
The shelter plasmid loss assay for strains carrying pHYD1613 and pHYD2373 (rne-169Q,ΔCTH) or only pHYD1613 (ΔrppH rne-ΔCTH) was done as described in the legend to Fig. 1. The strains used for the one-, four-, and five-operon deletions, respectively, were GJ16183, GJ16028, and GJ16030 (rne-169Q,ΔCTH) and GJ16184, GJ16124, and GJ16125 (ΔrppH rne-ΔCTH).