FIG 2.

Short N-terminal deletions in PduB result in faster growth of Salmonella on 1,2-propanediol minimal medium. The top panel is a schematic of N-terminal deletions that were tested. Residues highlighted in red indicate the deleted regions. Underlined residues correspond to a highly conserved putative α-helix. The pduBB′ deletion mutant is a control known to have a very high growth rate due to an MCP assembly defect. Other PduB deletion mutants are indicated in the legend. The M38A mutant changes the start codon of PduB′ and blocks its translation; thus, it is essentially a ΔpduB′ mutant. The table to the right shows the doubling time for each strain, and the error shown is one standard deviation determined from the results from three or more independent experiments. The wild type (WT) is Salmonella enterica LT2 serovar Typhimurium. OD₆₀₀, optical density at 600 nm.