FIG 4.

SDS-PAGE of MCPs purified from pduB deletion mutants with MCP assembly defects. WT, wild type. Letters on the right indicate the Pdu protein represented by each band. Lysozyme (lys) is not an MCP component but was used to lyse cells. The percent yield of purified MCPs indicates grams of protein/gram of cells relative to the wild type. The specific activities of the major lumen enzymes, the PduCDE diol dehydratase and the PduP aldehyde dehydrogenase, are shown in micromoles per minute per milligram. The error shown is one standard deviation determined from the results from three or more independent experiments. Ten micrograms of protein was loaded in each lane. Gels were stained using a Coomassie-based protocol. Enzyme assays are described in Materials and Methods.