FIG 5.

Individual point mutations along the highly conserved putative α-helix on the N terminus of PduB result in fast growth on 1,2-propanediol minimal medium. WT, wild type. The PduB Δ6–12 mutant is a control strain known to have a very high growth rate due to an MCP assembly defect. Four pduB mutants with site-directed mutations (V7T, I19T, M11S, and V14T) were also tested. The table to the right shows the doubling time for each strain, and the error shown is one standard deviation determined from the results from three or more independent experiments. Growth curves were determined with a microplate reader, as described in Materials and Methods.