TABLE 1.
Strains and plasmids
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| AH109 | Saccharomyces cerevisiae strain for two-hybrid analysis | Clontech |
| CB15N | Synchronizable derivative of CB15 | 66 |
| CJW3186 | CB15N ftsA::pXftsA | C. Jacobs-Wagner, unpublished data |
| CJW2959 | CB15N vanA::pVsigpepCHYN-4 | 53 |
| SP2 | CB15N ΔdipI::ΩSpc xylR::pXdipI5 | This study |
| SP3 | CB15N ΔdipI::ΩSpc xylR::pXdipItm5 | This study |
| SP4 | CB15N ΔdipI::ΩSpc xylR::pXdipItm5-vanR::pMT383 | This study |
| SP5 | CB15N ΔdipI::ΩSpc xylR::pXdipItm5-vanR::pVmCHYftsN6 | This study |
| SP6 | CB15N ΔdipI::ΩSpc xylR::pXdipItm5-vanR::pVdipMCHYC2 | This study |
| SP7 | CB15N ΔdipI::ΩSpc xylR::pXdipItm5-vanR::pVmreBswCHY4 | This study |
| SP8 | CB15N ΔdipI::ΩSpc xylR::pXdipItm5-murG::pmurGCHY4 | This study |
| SP9 | CB15N ΔdipI::ΩSpc xylR::pXdipItm5-vanR::pVmCHYftsI4 | This study |
| SP10 | CB15N ΔdipI::ΩSpc xylR::pXdipItm5-vanR::pVmCHYftsW6 | This study |
| SP12 | CB15N ΔftsN xylR::pXftsN5 | This study |
| SP13 | CB15N ΔftsI xylR::pXftsI5 | This study |
| SP14 | CB15N ΔftsL xylR::pXftsL5 | This study |
| SP15 | CB15N dipI::pdipICHY4 | This study |
| SP16 | CB15N ftsA::pXftsA2-dipI::pdipICHY4 | This study |
| SP17 | CB15N ΔftsL xylR::pXftsL5-dipI::pdipICHY4 | This study |
| SP18 | CB15N ΔftsI xylR::pXftsI5-dipI::pdipICHY4 | This study |
| SP19 | CB15N ΔftsN xylR::pXftsN5-dipI::pdipICHY4 | This study |
| SP20 | CB15N vanR::pVmCHYftsN6 | This study |
| SP21 | CB15N dipI::pdipICHY4-xylR::pXVENftsB2 | This study |
| SP22 | CB15N dipI::pdipICHY4-xylR::pXVENftsN2 | This study |
| SP23 | CB15N dipI::pdipICHY4-xylR::pXVENftsW2 | This study |
| SP24 | CB15N xylR::pXDipICHY5 | This study |
| SP25 | CB15N vanR::pVsp2DipICHY4 | This study |
| SP26 | CB15N dipI::pdipICHY4-xylR::pXVENftsI2 | This study |
| SP27 | CB15N ΔdipI::ΩSpc xylR::pXdipItm5-vanR::pVftsLCHY4 | This study |
| SP28 | CB15N ΔdipI::ΩSpc xylR::pXdipItm5-vanR::pVftsQCHY4 | This study |
| SP29 | CB15N ftsZ::pBJM1-dipI::pdipICHY4 | This study |
| XL1-Blue | Cloning strain | Invitrogen |
| Plasmids | ||
| pBGKT7 | Matchmaker system plasmid | Clontech |
| pADdipI | pGADT7 carrying dipI | This study |
| pADftsB | pGADT7 carrying ftsB | This study |
| pADftsI | pGADT7 carrying ftsI | This study |
| pADftsL | pGADT7 carrying ftsL | This study |
| pADftsN | pGADT7 carrying ftsN | This study |
| pADftsQ | pGADT7 carrying ftsQ | This study |
| pBDdipI | pBGKT7 carrying dipI | This study |
| pBDftsB | pBGKT7 carrying ftsB | This study |
| pBDftsI | pBGKT7 carrying ftsI | This study |
| pBDftsL | pBGKT7 carrying ftsL | This study |
| pBDftsN | pBGKT7 carrying ftsN | This study |
| pBDftsQ | pBGKT7 carrying ftsQ | This study |
| pBOR | pBluescript carrying a 2-kb EcoRI fragment from pHP45Ω | C. Stevens, unpublished data |
| pBJM1 | pBGST18 carrying 5′-terminal region of ftsZ fused to the xylXp promoter | 73 |
| pCHYC-2 | pMB1 replicon carrying mCherry | 74 |
| pdipICHY4 | pCHYC-4 carrying the 3′-terminal region of dipI fused to mCherry | This study |
| pGADT7 | Matchmaker system plasmid | Clontech |
| pMT383 | Integration vector with ftsZ-eyfp under the control of vanAp | 32 |
| pmurGCHY4 | pCHYC-4 carrying the 3′-terminal region of murG fused to mCherry | This study |
| pNPTDdipIW | pNPTS138 carrying ΔdipI::ΩSpc | This study |
| pNPTDftsI | pNPTS138 carrying ΔftsI | This study |
| pNPTDftsL | pNPTS138 carrying ΔftsL | This study |
| pNPTDftsN | pNPTS138 carrying ΔftsN | This study |
| pNPTS138 | pLitmus derivative carrying oriT and sacB | MRK Alley |
| pVCHYC-2 | pMB1 replicon carrying mCherry under the control of vanAp and vanR | 74 |
| pVCHYftsI4 | pVCHYN-4 carrying ftsI fused to mCherry | This study |
| pVCHYftsN6 | pVCHYN-6 carrying ftsN fused to mCherry | This study |
| pVchyftsW6 | pVCHYN-6 carrying ftsW fused to mCherry | This study |
| pVCHYN-6 | pMB1 replicon carrying vanR, vanAp, and mCherry | 74 |
| pVdipMCHY2 | pVCHYC-2 carrying dipM fused to mCherry | This study |
| pVmreBswCHY4 | pVCHYC-4 derivative carrying MreBswmCherry | This study |
| pXdipI5 | pXTCYC-5 derivative carrying dipI | This study |
| pXdipItm5 | pXTCYC-5 derivative carrying dipI fused with tmRNA degradation sequence | This study |
| pXftsI5 | pXTCYC-5 derivative carrying ftsI | This study |
| pXftsL5 | pXTCYC-5 derivative carrying ftsL | This study |
| pXftsN5 | pXTCYC-5 derivative carrying ftsN | This study |
| pXVENftsB2 | pXVENN-2 derivative carrying ftsB | This study |
| pXVENftsN2 | pXVENN-2 derivative carrying ftsN | This study |
| pXVENftsW2 | pXVENN-2 derivative carrying ftsW | This study |
| pXVENN-2 | pMB1 replicon carrying Venus under the control of xylXp | 74 |
| pXTCYC-5 | pMB1 replicon carrying xylR, xylXp, and tetracysteine tag | 74 |
| pVsp2CHYN-4 | pVCHYN-4 carrying dipMΔ109–609 | This work |
| pVsp2dipICHY4 | pVsp2CHYN-4 carrying dipIΔ1–33 | This work |
| pXdipICHY5 | pXCHYC-5 carrying dipI | This work |