TABLE 1.

Strains and plasmids used in this work

Strain or plasmida	Descriptionb	Source or reference
Strains
DL1 (Wt)	Wild type	66
DL100 (isogenic Wt)	Wild type; pilA Kanr	26
pilAY32F mutant	pilA contains the Tyr-32-Phe mutation (GCG TAC → GCA TTC)c; Kanr	This work
ΔpilA (in-frame deletion) mutant	In-frame deletion mutation; Kanr	26
Plasmids
pCD341	Source of Kanr cassette	74
pLC3	pilA (wild type) Specr	26
pLC9	pilA with Tyr-32-Phe mutation; Specr	This work

^aAll strains were G. sulfurreducens and were derived from DL1 (66).

^bKan^r, kanamycin resistance; Spec^r, spectinomycin resistance.

^cBoldface font denotes mutated bases.