Fig. 5.

Dietary restriction (DR) caused differential whole gene body methylation of lipid metabolism genes. a Differential methylation landscape of the Elovl6 gene locus at young and old age. Bins are represented as bars with color scale and height indicating methylation differences. Hatched bars indicate bins that show significantly different methylation between ad libitum (AL) and DR conditions. Arrows indicate gene orientation; merged mRNA structure is depicted below. b, c Scatterplot of differential expression versus whole gene body methylation differences at young (b) and old age (c). Number of genes per quadrant is denoted in corners; dashed lines indicate methylation difference cutoff of > ±2.5%. Fisher’s exact test indicates a significant (p < 0.032) inverse relationship (Pearson correlation −0.39) at old age but not at young age. d Gene ontology and reactome enrichment of genes with an inverse relationship of whole gene body methylation and differential expression at old age. Lengths of bars represent negative log-transformed, adjusted p values for Fisher’s exact enrichment test. Cells indicate log₂-fold changes (log2FC) between AL and DR per gene