Figure 2.

MiR-200a inhibited proliferation, migration and invasion of HCC cells. (A) Huh7 cells were transfected with miR-200a or negative control lentivirus, and qRT-PCR was performed to determine the expression of miR-200a. (B) Huh7 cells were transfected with miR-200a or negative control lentivirus, and proliferation ability of cells was detected by CCK8 testing kit. (C, D) Transwell assays of Huh7 cells transfected with miR-200a or negative control lentivirus were used to determine the migration and invasion (magnification×100, Nikon Te2000). Experiments were performed in triplicate. *P<0.05, **P<0.01, ***P<0.001, compared with the negative control (the negative control was Huh7 cells transfected with negative lentivirus in figure 2)