Table III.
Summary of porcine epidemic diarrhea virus (PEDV) real-time reverse transcription polymerase chain reaction (RT-PCR) results for rectal swabs collected on days 3 and 7 post-inoculation for the swine bioassay
| Treatment groupa | Day 3 post-inoculation; percentage positive for PEDV (positive/tested) | Day 7 post-inoculation; percentage positive for PEDV (positive/tested) | Bioassay result; percentage positive for PEDV (positive/tested)b |
|---|---|---|---|
| Negative control | 0% (0/3) | 0% (0/3) | 0% (0/3)a |
| Positive control | 100% (4/4) | 100% (4/4) | 100% (4/4)b |
| 5 mL-1:16 | 0% (0/4) | 0% (0/4) | 0% (0/4)a |
| 10 mL-1:16 | 0% (0/4) | 0% (0/4) | 0% (0/4)a |
| 5 mL-1:32 | 0% (0/4) | 0% (0/4) | 0% (0/4)a |
| 10 mL-1:32 | 0% (0/4) | 0% (0/4) | 0% (0/4)a |
| Transmission control | 25% (1/4) | 25% (1/4) | 25% (1/4) |
a
Treatment groups are summarized in Table I.
b
Values with different letters were significantly different (P < 0.05) by Fishers exact test. The transmission control group was included to validate the animal housing and handling protocols used for the bioassay. Values for transmission control group were not compared to those of the other groups.