Figure 5.

Flow cytometry results for the binding of His-ExPD-1-FITC (A), His-ExPD-L1-FITC (B), and FITC as a control with peripheral blood mononuclear cells (PBMCs) from pigs 7 d after experimental infection with Classical swine fever virus. The fluorescence signal of the protein binding was greater than the signal of the control binding for both proteins. In addition, PD-L1 on the surface of the PBMCs from the infected pigs was recognized by His-ExPD-1-FITC but not by FITC, and PD-1 on the surface of the PBMCs from the infected pigs was recognized by His- ExPD-L1-FITC but not by FITC. FL1-H — fluorescence height or intensity (relative units).