Fig. 1.

Prolonged leucine and serum deprivation increase REDD1 protein and mRNA expression. HeLa cells were deprived of leucine (A, C) or serum (B, D) for varying times up to 10 h. (A, B) Phosphorylation of p70S6K1 on T389 and REDD1 protein was assessed by Western blot analysis. REDD1 mRNA abundance (C, D) was assessed by SYBR Green qRT-PCR and normalized to GAPDH. Results represent the mean±SEM of 3 independent experiments with 2 replicates performed in each experiment. Experimental groups with means that are statistically different from one another are indicated with different letters above the bars (One-Way ANOVA and Tukey's post hoc test for multiple comparisons, p<0.05).