Fig. 5.

The revertant showed WT-like phenotypes. (A) Colony phenotypes of the WT, SCV, and revertant. (B) Growth curves of the WT, SCV, and revertant in AM3 medium at 37°C. (C) Biofilm formation by the WT, SCV, and revertant on microtiter plates after a 48-h incubation in AM3 medium at 37°C. The amounts of biofilms were normalized by cell densities (OD₆₀₀). Data are the average of at least six replicate wells and standard deviations are shown.