Fig 3. Pathway analysis illustrating the top ten canonical pathways affected by 15 mins of surgery.

A positive z-score suggests an increase in pathway activity, the darker the orange colour the higher the increase in pathway activity. Our data suggests that surgery increases signalling in the thrombin, aldosterone, B-cell, ERK-5, eNOS, iCOS-iCOSL signalling in T-helper cells and growth hormone signalling pathways. A grey colour indicates that axon guidance signalling and breast cancer signalling by stathmin-1 is perturbed by surgery but its direction is not-apparent. Values are derived by the change in relative expression compared with the control group (fold change). This data suggests that surgery affects endothelial function, neural plasticity and inflammatory processes.