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. 2017 Mar 29;12(3):e0174255. doi: 10.1371/journal.pone.0174255

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© 2017 Liao et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A) Alkaline Phosphatase Staining, (B) Alizarin Red Staining, (C) Von Kossa and (D) Toluidine Blue Staining, all of which analyze the differentiation of DMCs, indicate that osteogenic and chondrogenic differentiation is reduced in the Sirt6 KO group. (E) Oil Red O staining revealed an enhanced ability for adipogenic differentiation in the Sirt6 KO group.