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. 2017 Mar 29;12(3):e0174255. doi: 10.1371/journal.pone.0174255

Fig 7. SIRT6 activates the NF-κB/p65, p38-MAPK and ERK signaling pathways and thus affects cellular mitochondrial energy metabolism in DMCs.

Fig 7

(A) A western blot analysis of the Sirt6 KO group showed enhanced activation of the NF-κB/p65, p38-MAPK, and ERK signaling pathways on phosphorylation of those signaling molecules. Quantitative analysis obtained the same relative results. (B) PCR array analysis showed a significant difference between the KO and WT group with respect to the mRNA expression levels of Atp4b and Cox6a2. They were reduced by 51% and 54% in the KO group, whose ATP levels in the DMCs were significantly lower than those of the WT group. The gene expression level was normalized to GAPDH (n = 3). *p<0.05 versus CO. CO = control. GAPDH was used as a control.