. 2017 Mar 29;12(3):e0174255. doi: 10.1371/journal.pone.0174255

Table 1. Primer sequences and conditions for real-time polymerase chain reaction.

Gene	Sequence		Product bp
SIRT6	Forward	`CTGAGAGACACCATTCTGGACT`	188
SIRT6	Reverse	`GGTTGCAGGTTGACAATGACC`
Gapdh	Forward	`GGAGAGTGTTTCCTCGTCCC`	136
Gapdh	Reverse	`ATGAAGGGGTCGTTGATGGC`
Osx	Forward	`TGAGGAAGAAGCCCATTCAC`	198
Osx	Reverse	`ACTTCTTCTCCCGGGTGTG`
PPARΓ	Forward	`CAAGGTGCTCCAGAAGATGA`	60
PPARΓ	Reverse	`AGTAGCTGCACGTGCTCTGT`
Bsp	Forward	`ACCCCAAGCACAGACTTTTGA`	64
Bsp	Reverse	`TTTCTGCATCTCCAGCCTTCT`
OCN	Forward	`AGCAGGAGGGCAATAAGGTA`	108
OCN	Reverse	`CAAGCAGGGTTAAGCTCACA`
Bmp2	Forward	`GCTTTTCTCGTTTGTGGA`	162
Bmp2	Reverse	`TGGAAGTGGCCCATTTAGAG`
Ap2	Forward	`GCGTGGAATTCGATGAAATCA`	88
Ap2	Reverse	`CCCGCCATCTAGGGTTATGA`
ADD1	Forward	`TTTCAGAAGCAGCAGCGAGA`	119
ADD1	Reverse	`TCCTCTTTAGTCCACTTAGTCTTCG`
Adipoq	Forward	`ATCTGGAGGTGGGAGACCAA`	144
Adipoq	Reverse	`GGGCTATGGGTAGTTGCAGT`