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. 2017 Mar 29;12(3):e0174150. doi: 10.1371/journal.pone.0174150

Table 1. Genetic diversity detected in 367 D. alata accessions using 24 microsatellite markers.

Ho, He and Fis values were quantified only for diploids.

Origin 1 SSR EMBL2 Motif Min.–max. size (bp) Total alleles Al < 1%3 Main allele frequency4 Ho5 He6 Fis7
D. A Da3G04 AJ880369 (AC)12 282–306 9 1 0.80 0.83 0.86 0.03
D. A Da1F08 AJ880368 (TG)13 161–185 9 3 0.88 0.32 0.39 0.19
D. A Da2F10 [51] (TG)14 108–151 14 2 0.46 0.65 0.67 0.04
D. A Da1A01 AJ880381 (GT)8 201–222 7 1 0.85 0.52 0.51 -0.02
D. AB Dab2D11 [51] (TC)19 227–247 9 2 0.66 0.83 0.67 -0.25*
D. PR Dpr3E10 [51] (TCT)13(CTC)4 170–194 10 1 0.84 0.16 0.32 0.16*
D. PR Dpr3B12 AJ880376 (TG)8 132–150 8 2 0.81 0.74 0.65 -0.15
D.J DIJ034 AB201419 (AG)17 198–267 15 2 0.48 0.90 0.80 -0.14
D.J DIJ443 AB201420 (AG)17 257–285 12 0 0.45 0.73 0.82 0.11*
D.J DIJ0461 AB201423 (GA)16 120–144 8 3 0.64 0.78 0.71 -0.10
D.J DIJ1045 AB201422 (TG)19 251–281 13 4 0.52 0.78 0.71 -0.10
D.A mDaCIR2 FN677762 (CA)10 243–268 9 0 0.44 0.78 0.71 -0.10
D.A mDaCIR11 FN677767 (AC)10 165–183 8 4 0.79 0.47 0.54 0.12*
D.A mDaCIR13 FN677768 (GA)17 186–214 14 4 0.49 0.56 0.79 0.28*
D.A mDaCIR17 FN677770 (AC)7 228–236 5 2 0.96 0.18 0.20 0.11*
D.A mDaCIR20 FN677773 (GA)16 172–206 11 1 0.69 0.67 0.62 -0.08
D.A mDaCIR26 FN677776 (TG)15(GA)14 171–219 12 2 0.59 0.60 0.74 0.18
D.A mDaCIR57 FN677784 (TG)9 143–152 5 0 0.86 0.83 0.55 -0.28*
D.A mDaCIR59 FN677786 (TC)11(CA)9 186–224 12 5 0.55 0.75 0.75 -0.01
D.A mDaCIR60 FN677787 (CA)11 132–159 12 1 0.56 0.62 0.81 0.23
D.A mDaCIR25 [38] (AC)14 142–184 14 1 0.39 0.49 0.81 0.34*
D.A mDaCIR61 FN677788 (AG)21 179–221 18 5 0.61 0.81 0.74 -0.09
D.A mDaCIR63 [38] (AG)12 155–180 8 1 0.68 0.53 0.65 0.18*
D.A mDaCIR116 FN677800 (AG)8(AG)7 83–126 14 3 0.70 0.61 0.65 0.06*

1D. A, D. alata; D. AB, D. abyssinica; D. PR, D. praehensilis; D. J, D. japonica

2 Registration number on EMBL database or publication reference

3 Rare alleles with a frequency lower than 1%

4 Highest frequency of an allele observed at this locus

5 Observed heterozygosity.

6 Expected heterozygosity

7 Fixation index,*P <0.001