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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: Mol Cell Endocrinol. 2016 Dec 8;442:81–89. doi: 10.1016/j.mce.2016.12.002

Fig 2. Decrease in the association of LRBP and LHCGR mRNA during FSH+E2-mediated LHCGR induction.

Fig 2

Granulosa cells were isolated from immature female rats injected subcutaneously with estradiol (1.5 mg) for three consecutive days. Cells were cultured in serum-free media for 24 hours and then treated with FSH (50 ng/ml) and E2 (1nM) for different time periods. S100 fractions were isolated as described in Section 2.3. Gel mobility shift analysis was performed using S100 fractions containing equal amounts of total protein after incubating with [32P]-labeled LBS (1.5 x 105 c.p.m). The autoradiogram shown is representative of three independent experiments. Densitometric scanning of the bands is represented in the bar diagram. Error bars represent mean ±SE. *p<0.05 vs. control; n=4.