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. Author manuscript; available in PMC: 2018 Feb 15.

Published in final edited form as: Mol Cell Endocrinol. 2016 Dec 8;442:81–89. doi: 10.1016/j.mce.2016.12.002

Fig 3 — Granulosa cells were isolated from immature female rats injected subcutaneously with estradiol (1.5 mg) for three consecutive days. Cells were cultured in serum-free media for 24 hours and then pretreated with four different doses of AdmiR-122 (1 x 10⁶, 1 x 10⁷, 1 x 10⁸, 1 x 10⁹ pfu) for 24 (A) or 48 (B) hours. Total RNAs were isolated and reverse transcribed, and the resulting cDNAs were subjected to real-time PCR quantitation using specific primers and probes for miR-122. The graph represents changes in mRNA levels normalized to U6 snRNA and shown as fold-change vs. control. Error bars represent mean ±SE. *p<0.05 vs. control; n=4.

Fig 3 — Granulosa cells were isolated from immature female rats injected subcutaneously with estradiol (1.5 mg) for three consecutive days. Cells were cultured in serum-free media for 24 hours and then pretreated with four different doses of AdmiR-122 (1 x 10⁶, 1 x 10⁷, 1 x 10⁸, 1 x 10⁹ pfu) for 24 (A) or 48 (B) hours. Total RNAs were isolated and reverse transcribed, and the resulting cDNAs were subjected to real-time PCR quantitation using specific primers and probes for miR-122. The graph represents changes in mRNA levels normalized to U6 snRNA and shown as fold-change vs. control. Error bars represent mean ±SE. *p<0.05 vs. control; n=4.