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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: Mol Cell Endocrinol. 2016 Dec 8;442:81–89. doi: 10.1016/j.mce.2016.12.002

Fig 4. Overexpression of miR-122 inhibited LHCGR upregulation.

Fig 4

Cells were pretreated with AdmiR-122 (1 x 109 pfu) or AdGFP (1 x 109 pfu) for 24 hours. Media was replaced after 24 hours and cells were treated with FSH (50 ng/ml) and E2 (1nM) for six hours. Total RNAs were isolated and reverse transcribed, and the resulting cDNAs were subjected to real-time PCR quantitation using specific primers and probes for LHCGR. The graph represents changes in mRNA levels normalized to 18S rRNA and shown as fold-change vs. control. Error bars represent mean ±SE. *p<0.05 vs. AdGFP; **p<0.05 vs. AdGFP+FSH+E2; n=4.