Table 4. Mortality rate and cumulative intake in dead and surviving bees in different experimental setups for temporary¹ (T), acute² (A), permanent³ (P) and chronic⁴ (C) feeding regimes.

Imidacloprid, duration of experiments, bee mortality, median food intake of dead and surviving bees and the respective p-value for the comparison of median intake of dead and surviving bees (Mann-Whitney U-test) for all experiments are shown. For number of replicates see Table 1.

Experiment	Imidacloprid	Duration	Bee mortality (%)	Median food intake per bee (μL)
				dead	surviving	p-value
10 bees per cage
T1	-	3–7	5.0	0.9	9.7	0.00
T3	-	48	0	-	2.2	-
T4	-	48	0	-	10.6	-
T5	-	48	16.7	2.0	10.9	0.00
T6	-	48	5	0.4	10.0	0.00
T7	-	48	1.7	-	9.7	-
T8*	-	48	13.3	0.8	9.0	0.00
T9	-	48	16.7	0.4	10.9	0.00
T10	-	48	6.7	0.5	10.4	0.00
T11	-	48	6.7	0.9	11.0	0.00
P1	-	48	11.7	9.2	44.7	0.00
P2	-	48	0	-	37.9	-
P3	-	48	6.7	0	30.8	0.00
P4	-	72	1.7	-	68.0	-
P5	-	72	6.7	1.7	57.5	0.00
A1	LD50	3–7	25.0	0.6	10.4	0.00
A2	LD50	48	55.0	7.1	10.1	0.29
A3	1/50 LD50	48	6.7	0.3	9.6	0.00
C1	LC50	48	18.3	15.9	19.1	0.17
C2	LC50	48	25.0	5.1	20.7	0.00
C3	LC50	48	20.0	9.7	16.0	0.06
C4	1/50 LC50	48	1.7	-	21.9	-
30 bees per cage
T14	-	3–7	0	-	1.5	-
T15	-	48	16.7	1.5	3.1	0.00
P6	-	48	0	-	28.9	-
A5	LD50	48	12.2	1.6	2.6	0.17
C5	LC50	48	5.6	6.0	19.4	0.00
C6	LC50	48	23.3	14.2	21.4	0.00

*12 bees per cage

¹ temporary: test bees initially provided with defined amount (25μL/100μL) of ¹⁴C labelled diet, followed by unlabelled diet ad libitum for maintenance

² acute: test bees initially provided with defined amount (25μL/100μL) of ¹⁴C labelled diet containing LD₅₀ or ¹/₅₀ LD₅₀ imidacloprid, followed by unlabellied diet ad libitum for maintenance

³ permanent: test bees provided with ¹⁴C labelled diet ad libitum

⁴ chronic: test bees provided with ¹⁴C labelled diet containing LD₅₀ or ¹/₅₀ LD₅₀ imidacloprid ad libitum