Skip to main content
. 2017 Mar 17;13(3):e1006280. doi: 10.1371/journal.ppat.1006280

Fig 4. MS Analysis of LPS from G27 HP1284 and waaL Deletion Mutants.

Fig 4

The LPS samples were methanolysed, permethylated and analysed by MS. MALDI-TOF spectra of LPS from G27 HP1284 and waaL deletion mutants are shown in (A) and (B), respectively. The MS peaks corresponding to sodiated heptan-glucan structures are coloured red and annotated with m/z values. Most blank peaks are due to contamination and the addition of a sodium atom. The MS data indicate the core-oligosaccharide of G27 LPS is a hexa-saccharide with a sequence of Glc-Gal-Hep-Hep-Hep-KDO. The deletion of HP1284 leads to an incompletely synthesized core, which does not affect its O-antigen.