Fig. 1.

Consort statement and methodology. *High-risk factors in infertile patients, who were subjected to tests of ovarian reserve, were age >35 years, previous ovarian surgery, family history of premature ovarian failure, poor responders to standard ovulation induction protocol, with clomiphene or gonadotrophins, suspected unexplained infertility. ^#DOR: Any one criteria for diagnosis of diminished ovarian reserve (DOR) in cases of infertility were [4, 6–12]. (1) Age-specific FSH levels using the ELFA technique (enzyme-linked fluorescent assay) (>7 mIU/ml for age <33 years; >7.9 mIU/ml for age 33–37 years; >8.4 mIU/ml for age >38 years). (2) AMH levels done using the AMH Gen II enzyme-linked immunosorbent assay <1.05 ng/ml. (3) Sonographic antral follicle count AFC ≤ 4. The diameter of the follicle in the range of 2–10 mm was counted in both ovaries and added to give total antral follicle count