Skip to main content
. 2017 Mar 22;5(6):e13175. doi: 10.14814/phy2.13175

Figure 3.

Figure 3

Overexpression of arginase II increased viable cell numbers. HeLa cells were transfected with an adenoviral vector containing either the human arginase II gene (AdArg2) or the gene for green fluorescent protein (AdGFP). (A) Transfection substantially increased arginase II protein levels. Representative western blot for arginase II from cells transfected with either AdGFP or AdArg2 and incubated in either normoxia or hypoxia for 24 h. (B) Cells were transfected with either AdGFP or AdArg2 and 24 h later the cells were washed and fresh media placed on them for a 24 h recovery period. The cells were then trypsinized and equal numbers of cells plated in each well of a 6‐well plate. The cells were then exposed to either normoxia or hypoxia for 48 h and viable cells counted using trypan blue exclusion (n = 3 for each group). * AdArg2 different from AdGFP same exposure, P < 0.001. # hypoxia different from normoxia same treatment, P < 0.001.