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. 2017 Mar 22;5(6):e13175. doi: 10.14814/phy2.13175

Figure 5.

Figure 5

EGF increased arginase II expression and viable cell numbers in HeLa cells. (A) Representative western blot for arginase II, cells were incubated in normoxia for 24 h with either no treatment or EGF (10, 20, or 100 ng) added to the media. Addition of EGF resulted in easily detectable arginase II protein levels in normoxic HeLa cells. (B) The EGF‐induced increase in arginase II was prevented by the small molecule EGFR inhibitor AG1478. Representative western blot for arginase II, cells were incubated in normoxia for 24 h with either no treatment or EGF (10 ng) added to the media and either vehicle (0 μmol/L AG1478) or increasing concentrations of AG1478 (1, 10, or 20 μmol/L). Addition of AG1478 prevented the EGF‐induced increase in arginase II protein levels in normoxic HeLa cells. (C) EGF increased viable cell numbers, an effect that was prevented by treatment with AG1478. Equal numbers of HeLa cells were plated in each well of 6‐well plates. Cells (n = 3 in each group) were either untreated or treated with 10 ng EGF, cells were also treated with either vehicle or increasing concentrations of AG1478. After incubating in normoxia for 48 h viable cells were counted using trypan blue exclusion. * EGF different from control, P < 0.05. # 10 μmol/L AG1478 treatment different from EGF alone, P < 0.01. $ 20 μmol/L AG1478 different from EGF alone, P < 0.001.