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. 2017 Mar 22;5(6):e13175. doi: 10.14814/phy2.13175

Figure 6.

Figure 6

EGF neutralizing antibody (EGF Ab) prevented the hypoxia‐induced increase in viable cell numbers. (A) EGF mRNA was substantially greater in hypoxic HeLa cells than in normoxic cells (n = 3 in each exposure). ** hypoxia different from normoxia, P < 0.0001. (B) Neutralizing EGF prevented the hypoxia‐induced increase in viable cell numbers. Equal numbers of HeLa cells were plated in each well of a 6‐well plate. Cells (n = 3 in each group) were treated with 3 μg of either IgG or EGF Ab and placed in either normoxia or hypoxia for 48 h. Viable cells were counted using trypan blue exclusion. * EGF Ab different from control in same exposure, P < 0.001. # hypoxia different from normoxia, P < 0.001.