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Pyruvate dehydrogenase (PDH) flux versus PDH and PDK4 protein level and PDH phosphorylation in isolated mitochondria from rat skeletal muscle. (A) Total PDH (PDH1Eα). (B) Degree of PDH phosphorylation (PDH‐P/PDH). (C) Level of Pyruvate dehydrogenase kinase 4 (PDK4). (D) The ratio (PDH oxidation rate(/(β‐oxidation rate). All Western blotting data are given in arbitrary units.
