Table 1.

Biochemical parameters

	Effect of Achilles tendon	Effect of ND
CML (nmol/mmol hydroxyproline)	14.9 ± 0.7***	7.4 ± 1.4**
CEL (nmol/mmol hydroxyproline)	10.5 ± 0.9***	0.6 ± 0.9
MG‐H1 (nmol/mmol hydroxyproline)	177.4 ± 13.6***	39.6 ± 10.1**
Pentosidine (within Tail) (nmol/mmol hydroxyproline)		0.010 ± 0.005
Pentosidine (within Achilles) (nmol/mmol hydroxyproline)		0.031 ± 0.005***
Pentosidine (within ND) (nmol/mmol hydroxyproline)	0.056 ± 0.005***
Pentosidine (within HFD) (nmol/mmol hydroxyproline)	0.035 ± 0.005***

Mean ± SE for each group. Mean difference for effects of Achilles tendon and ND. Significant difference (**P < 0.001, ***P < 0.0001).

For pentosidine there was an interaction (P < 0.01), which showed a significant increase in pentosidine for the ND group in the Achilles (P < 0.0001), but not in the tail tendon (P = 0.27). For both diets there was an effect of tissue (P < 0.0001).

HFD, High‐Fat Diet; ND, Normal Diet.