FIGURE 1.

¹H-NMR (A) and ¹³C-NMR (B) spectra of water-methanol fraction of chloroform- methanol extract of the dormant ovoid Mycobacterium smegmatis cells. Dormant cells were obtained after M. smegmatis cell growth in modified Sauton medium under gradual acidification (14 days of incubation) as described in M&M. Cells were centrifuged, washed and extracted by chloroform-methanol (for details see M&M). Duplet at 5.18–5.19 ppm in ¹H-NMR (A) spectrum and peak at 95.96 ppm in ¹³C-NMR (B) belong to anomeric proton and to anomeric C-1 atom in trehalose molecule correspondently. For description of other peaks in each spectrum (see Results).