FIGURE 2.

Accumulation of trehalose during transition of M. smegmatis cells to dormancy. M. smegmatis cells were cultivating under gradually acidification as described in M&M accompanied by accumulation of dormant ovoid cells. Periodically cell samples were withdrawn and water-methanol extracts were prepared. The extracts were analyzed by TLC (A) or by ¹H-NMR (B). Plates were stained by 10% H₂SO₄. Calculation of trehalose percentage on the basis ¹H-NMR (closed bars, B) was done as describe in M&M. Incorporation of ³H-uracil in cells is represented as CPM in 0.2 ml of culture (open circles, B). Bars represent ± SD.