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. 2017 Mar 30;8:524. doi: 10.3389/fmicb.2017.00524

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Copyright © 2017 Shleeva, Trutneva, Demina, Zinin, Sorokoumova, Laptinskaya, Shumkova and Kaprelyants.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Viability of dormant M. smegmatis cells correlates with intracellular trehalose content. Dormant M. smegmatis cells contained empty pES plasmid (open symbols) and cells with over-expressed trehalase MSMEG_4535 (closed symbols) were obtained and kept under static conditions as describe in M&M. Periodically samples were withdrawn for trehalose level determination (triangles) and viability estimation by MPN assay (circles). Zero time corresponds to the time of transferring of 13–15 days old post-stationary phase cells to plastic capped tubes. This experiment was performed in three biological repeats. A typical result is shown. Bars represent (95%) confidence limits for the MPN assay. Bars for trehalose content represent ± SD.