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. 2017 Mar 30;8:461. doi: 10.3389/fpls.2017.00461

FIGURE 5.

FIGURE 5

The emergence of differential regulation of PIN expression in the fiber indicates its important role in fiber elongation during the evolution of cotton. (A) Comparisons of mature fibers of G. hirsutum, G. arboreum, and G. raimondii. Bar = 1.5 cm. (B) Final statistics of mature fibers of G. hirsutum, G. arboreum, and G. raimondii. Each data is the mean of three independent experiments with a total of 10 measured samples. The error bars indicate the mean values ± SE. (C) Quantitative RT-PCR analysis of the GaPIN6 and GaPIN8 genes. ∗P < 0.05. (D) Dot plots showing consensus in the promoter regions of GaPIN6 (left) and GaPIN8 (right) and their orthologs from the At subgenome. T, putative TATA box; AuxRE, putative auxin response element; P box, putative pyrimidine box. (E) Quantitative RT-PCR analysis of the PIN genes using the Dt (indicated by blue lines) or D-originated (red lines) copies at the 10 DPA as the template. Three biological replicates were analyzed via qRT-PCR in (C,E), and the error bars represent the mean values ± SE. The expression levels are relative to cotton UBQ7. DPA, day after anthesis. ∗∗∗P < 0.001. (F) Promoter sequence analysis of GrPIN1a and its orthologs from the Dt subgenome. The blue box marks the putative HD-ZIP binding site, and the red color represents the putative ZF-HD binding site.