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. 2017 Mar 30;8:396. doi: 10.3389/fpls.2017.00396

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Copyright © 2017 Xie, Liu, Zhang, Tang, Yin, Fan, Zhu, Han, Song and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization and transcriptional activation analysis of PvPCF5. (A) Subcellular localization of PvPCF5 was performed in switchgrass protoplasts. Confocal images showing the PvPCF5-GFP fusion protein driven by the 35S promoter was specifically expressed in the protoplasts. 35S::GFP was used as a control. Bar = 10 μm. (B) Transactivation activity of the PvPCF5 protein in yeast. The full-length, N-terminal region, C-terminal region of the PvPCF5 were fused into DNA sequences containing a GAL4 DNA-binding domain with the negative control plastid (pGBKT7). These four kinds of constructs were expressed in the yeast strain Y2HGold. The transformants were streaked on the SD/-trp and SD/-trp/A/X-α-gel plates, plates were cultured for 2–4 days at 30°C to check a possible transcriptional activation function of PvPCF5.