Table 2.
PANTHER classification for the HARS groups with and without pancytopenia
| PANTHER classification | HARS without pancytopenia | HARS with pancytopenia | ||||
|---|---|---|---|---|---|---|
| Upregulated/downregulated | Over/under repres. | p values | Upregulated/downregulated | Over/under repres. | p values | |
| Biological process | ||||||
| B cell-mediated immunity | Down/up | ++ | 1.9E−06/1.3E−07 | Down/up | ++ | 5.2E−05/3.5E−07 |
| Natural killer cell activation | Up | + | 4.3E−08 | Down/up | ++ | 2.6E−04/3.1E−07 |
| Immune response | Down/up | ++ | 3.4E−04/4.6E−11 | Up | + | 3.3E−09 |
| Immune system process | Up | + | 1.9E−07 | Up | + | 5.0E−08 |
| Cell communication | Up | + | 9.4E−06 | Up | + | 8.0E−08 |
| Cellular process | Up | + | 5.7E−07 | Up | + | 4.0E−07 |
| Response to stimulus | Up | + | 7.6E−06 | Up | + | 5.9E−06 |
| Translation | Down/up | +− | 5.5E−04/5.1E−06 | Up | − | 1.0E−05 |
| Cell death | Up | + | 5.7E−05 | Up | + | 3.4E−05 |
| Apoptotic process | Up | + | 5.7E−05 | Up | + | 3.4E−05 |
| Death | Up | + | 6.3E−05 | Up | + | 3.7E−05 |
| mRNA processing | Up | − | 3.8E−05 | |||
| RNA metabolic process | Up | − | 8.5E−05 | |||
| Organelle organization | Down | + | 3.7E−04 | |||
| Carbohydrate metabolic process | Down | - | 7.1E−04 | |||
| Macrophage activation | Up | + | 2.5E−05 | |||
| Protein phosphorylation | Up | + | 6.2E−05 | |||
| Regulation of catalytic activity | Up | + | 9.7E−05 | |||
| Protein class | ||||||
| Cytokine receptor | Up | + | 8.6E−08 | Down/up | ++ | 3.6E−04/4.7E−07 |
| Enzyme modulator | Up | + | 4.9E−05 | + | 3.0E−06 | |
| RNA binding protein | Up | − | 2.9E−08 | − | 8.9E−06 | |
| G-protein modulator | Up | + | 1.8E−05 | + | 8.0E−05 | |
| Ribosomal protein | Down | + | 9.0E−09 | |||
| Nucleic acid binding | Up | − | 4.9E−05 | |||
| Defense/immunity protein | Up | + | 4.6E−06 | |||
| Immunoglobulin receptor superfamily | Up | + | 7.3E−05 | |||
| mRNA processing factor | Up | − | 9.6E−05 | |||
| Molecular function | ||||||
| Protein binding | Up | + | 8.4E−09 | Up | + | 2.3E−11 |
| RNA binding | Up | − | 8.5E−08 | Up | − | 1.7E−06 |
| Nucleic acid binding | Up | − | 2.1E−05 | Up | − | 2.8E−05 |
| Small GTPase regulator activity | Up | + | 4.1E−06 | Up | + | 4.9E−05 |
| Structural constituent of ribosome | Down | + | 3.2E−06 | |||
| mRNA binding | Up | − | 3.5E−05 | |||
| Ion channel activity | Down | − | 4.2E−04 | |||
| Pathway | ||||||
| Inflammation mediated by chemokine and cytokine signaling pathway | Up | + | 4.5E−07 | Up | + | 1.5E−09 |
| Toll receptor signaling pathway | Up | + | 4.2E−05 | Up | + | 1.1E−07 |
| Pentose phosphate pathway | Up | + | 6.6E−05 | |||
| Integrin signaling pathway | Up | + | 5.3E−05 | |||
| Apoptosis signaling pathway | Up | + | 4.6E−04 | |||
Using the overlapping number of DEG from day 1 and day 2 after exposure for HARS groups with and without pancytopenia, a classification of overrepresented and underrepresented genes coding, e.g., biological processes or protein classes, was conducted using the bioinformatic tool PANTHER (http://www.pantherdb.org; version 10.0) which comprises Gene Ontology (GO) annotations directly imported from the GO database. Based on the comparison of observed vs expected numbers of upregulated or downregulated genes (reference database was Homo sapiens) for biological processes, e.g., “immune system process,” an overrepresentation (+) or underrepresentation (−) in the number of genes annotated to this process and a corresponding p value (Bonferroni corrected) was calculated. Numbers in italics refer to processes which differ among both HARS groups