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. 2017 Mar 30;8:473. doi: 10.3389/fpls.2017.00473

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Copyright © 2017 Shivakumara, Chaudhary, Kamaraju, Dutta, Papolu, Banakar, Sreevathsa, Singh, Manjaiah and Rao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Southern blot for T₀ eggplant transformants harboring the Host-induced gene silencing (HIGS) construct of msp-18 (A) and msp 20 (B) genes. Probes used for hybridization were specific to msp-18 and msp-20 genes. Untransformed control (UC) and wild-type (WT) plants did not show any hybridization signal to either of the probes. M: Lambda HindIII digest marker.