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. 2017 Mar 30;8:528. doi: 10.3389/fmicb.2017.00528

Figure 1.

Figure 1

Gene organization of the T6SS clusters in V. fluvialis in comparison with V. furnissii, V. cholerae, and V. splendidus. (A) Genes encoding VflT6SS1 in V. fluvialis 85003 were named according to the Tss nomenclature (tssA1, tssB1, tssC1, tssD1, tssE1, tssF1, tssG1, tssH1, tssJ1, tssK1, tssL1, tssM1, tagH1) or by their usual vernacular names (paar, impD, and rhs). Homologous genes are colored similarly. Open reading frames with unknown functions are shown in white. The lines below the colored genes indicate the loci amplified by PCR in the different isolates. (B) Genes encoding VflT6SS2 in V. fluvialis 85003 were names according to the Tss nomenclature (tssA2, tssB2, tssC2, tssE2, tssF2, tssG2, tssH2, tssI2, tssJ2, tssK2, tssL2, tssM2, tagH2) or vernacular names (vasH, vasI, tsiI2, and vasL). The 17 genes show high conservation with the well-characterized T6SS of V. cholerae N16961. The counterparts of V. fluvialis 33809 and V. furnissii NCTC11218 exhibit the identical gene organization in their respective chromosomes. The lines below the colored genes indicate the loci amplified by PCR in the different isolates. Three “orphan” hcp-vgrG alleles were designated tssD2_a-tssI2_a, tssD2_b-tssI2_b, and tssD2_c-tssI2_c, respectively. The homolog of tssD2_c-tssI2_c is absent in V. fluvialis 33809, V. furnissii NCTC11218, and V. cholerae N16961.