Table 1.

Biomass analysis of ZmDof1 wheat events.

N trt	Line	Shoot DW		Root DW		Total DW		NUE
		gr	% WT	gr	% WT	gr	% WT	Index	% WT
High N	WT	3.4 ± 0.5b		0.8 ± 0.2b		4.3 ± 0.6b		0.28 ± 0.04a
	UD-1	2.4 ± 0.4c	69	0.5 ± 0.1bcd	64	2.9 ± 0.5c	68	0.19 ± 0.04a	68
	UD-2	2.3 ± 0.5c	65	0.5 ± 0.1cd	61	2.7 ± 0.6c	65	0.18 ± 0.04a	65
	UD-3	1.8 ± 0.2c	54	0.4 ± 0.1d	49	2.2 ± 0.3c	53	0.15 ± 0.02a	53
	RD-1	3.0 ± 0.4b	88	0.8 ± 0.2bc	100	3.8 ± 0.6b	90	0.26 ± 0.04a	90
	RD-2	4.1 ± 0.5a	119	1.1 ± 0.2a	141	5.2 ± 0.7a	123	0.35 ± 0.05a	123
	RD-3	4.3 ± 0.6a	124	1.2 ± 0.2a	152	5.5 ± 0.8a	129	0.37 ± 0.05a	129
Low N	WT	1.8 ± 0.2ab		0.6 ± 0.1b		2.3 ± 0.3abc		7.8 ± 1.11b
	UD-1	1.6 ± 0.2ab	91	0.5 ± 0.1bcd	88	2.1 ± 0.3bc	90	7.0 ± 0.86bc	90
	UD-2	1.4 ± 0.3b	81	0.5 ± 0.1cd	85	1.9 ± 0.4c	82	6.4 ± 1.44bc	82
	UD-3	1.3 ± 0.2b	73	0.4 ± 0.1d	62	1.6 ± 0.2c	70	5.5 ± 0.69d	70
	RD-1	1.6 ± 0.2ab	91	0.6 ± 0.1bc	95	2.2 ± 0.4bc	92	7.2 ± 1.22b	92
	RD-2	2.3 ± 0.3a	128	0.8 ± 0.1a	137	3.1 ± 0.4a	130	10.2 ± 1.44a	130
	RD-3	2.2 ± 0.2a	123	0.7 ± 0.1a	123	2.9 ± 0.3ab	123	9.6 ± 0.88a	123

Shoot dry weight, root dry weight, total dry weight, and nitrogen use efficiency (NUE, Dry biomass per N supplied) of control plants (WT). gr refers to grams; three UBI4/Dof1 transgenic events (UD-1, UD-2, UD-3); and three rbcS1/Dof1 transgenic events (RD-1, RD-2, RD-3). Plants were grown hydroponically for 5 weeks under 15 mM N (High N) or 0.3 mM N (Low N). Data expressed as mean ± standard error from three independent experiments. Different letters indicate statistical differences (p < 0.05) within N trt.