Figure 10.

Glucose starvation induces NapA nuclear localization. NapA does not accumulate in nuclei during growth in arabinose or fructose as sole carbon sources but it does accumulate in nuclei during glucose starvation. Conidia from strain CAM20 (NapA::GFP) were grown for 18 h in MM at 37°C and then mycelia was shifted to MM with or without glucose or with fructose or glycerol as carbon source for indicated times (0–120 min). Mycelial samples were observed in vivo and photographed every 60 min using Epifluorescence microscopy. Lower panel shows nuclei (DAPI) and NapA::GFP fluorescence in mycelia starved for glucose during 120 min, fixed and photographed. Larger square areas in each picture show enlargements of the areas indicated by smaller squares.