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. 2017 Mar 30;8:516. doi: 10.3389/fmicb.2017.00516

Figure 2.

Figure 2

Transcriptional factors NapA, SrrA and AtfA play differential roles in catalase regulation. (A) CatA activity levels are not affected in ΔnapA conidia. Conidial protein extracts from strains CLK43 (WT), TFLΔatfA-04 (ΔatfA) and CFL7 (ΔnapA) were analyzed for catalase in-gel activity. (B) CatB activity induction by H2O2 requires NapA. Mycelia from the strains CLK43 (WT) and CFL7 (ΔnapA) were grown for 12 h in liquid MM and then H2O2 was added at the indicated times and concentrations and protein extracts were used for catalase activity determination. (C) NapA, AtfA, and SrrA are not required for CatB induction during the stationary phase of growth. Mycelia from the strains CLK43 (WT), CFL7 (ΔnapA), TFLΔatfA-02 (ΔatfA), COSsrrA3 (ΔsrrA) and TLK12 (ΔcatB) was grown for 12, 24, and 48 h in liquid MM medium. The experiment was repeated at least 3 times; a representative experiment is shown.