Figure 8.

NapA is required for the ethanol utilization and for resistance to Allyl alcohol. (A) Conidia (1 × 10³) from strains CLK43 (WT), CFL7 (ΔnapA) and MH440 (creA^d204) were inoculated on supplemented MM plates containing either 1% glucose, 1% ethanol or 1% glucose plus 5 mM Allyl alcohol (AA) and were incubated at 37°C for 4 days. (B) Mycelial plugs cut from the growing edge of 5-day colonies from the strains in (A) were transferred to the indicated media and incubated at 37°C for 4 days. (C) Conidia (1 × 10³) from strains CLK43 (WT), CFL7 (ΔnapA), CAM17 (ΔalcA), and CAM18 (ΔalcA ΔnapA) were inoculated on supplemented MM plates containing either H₂O₂ at the indicated concentrations or 1% glucose (MM), 1% ethanol or 1% glucose plus 5 mM allylic alcohol (AA) and incubated at 37°C for 4 days.