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. 2016 Dec 22;6(1):1. doi: 10.3390/plants6010001

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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RNA extraction from 4-day sprouted seed. Upper panel: homogenate solidification during RNA extraction by GeneJet minikit using the standard protocol. (A) An inverted microcentrifuge tube showing lysis buffer at the bottom; (B) a tube showing the solidified homogenate. Middle panel: incomplete phase separation in the standard TRIzol method; (C) a microcentrifuge tube containing TRIzol reagent; (D) a tube showing the hazy phase separation of the homogenate. Lower panel: expected phase separation in the Li and Trick [11] method; (E) a microcentrifuge tube showing an upper aqueous phase, an interphase (phenol-chloroform phase) in the middle, and a lower solid phase at the bottom; (F) a tube in inverted orientation following removal of the upper aqueous phase showing shiny starch granules deposited at the bottom.