Skip to main content
. 2016 Dec 22;6(1):1. doi: 10.3390/plants6010001

Table 1.

RNA quality and yield from the GeneJet, TRIzol, Li and Trick [11] and Wang et al. [12] methods. Standard: extraction after grinding in liquid N2; modified: extraction without liquid N2 by grinding in the extraction buffer; N/A; not applicable.

Absorbance Ratios
A260/A280 * A260/A230 *
Extraction method Before–After
DNase I Treatment
Before–After
DNase I Treatment
RNA per Seed μg/48 mg *
GeneJet Column N/A N/A 0.0
TRIzol method (standard) 1.85c–1.87b 0.57d–0.61c 1.1b
Li and Trick [11] (standard) 1.90bc–2.10a 0.80c–2.20a 12.9a
Li and Trick [11] (modified) 2.06a–2.05a 1.32a–2.12a 14.2a
Wang et al. [12] (modified) 1.99ab–2.08a 1.05b–1.86b 14.6a

* Bonferroni t-tests (Means with the same letter are not significantly different).