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Detection of hydrogen peroxide using DAB staining in Vigna unguiculata leaves exposed to the following treatments: Control (C), Drought (D), Ozone (O), Ozone + Drought (OD). Leaf discs were collected and stained after 14 days of treatment. Pictures are representative of three independent biological replicates. No specific staining was observed in control leaves treated with ascorbic acid (data not shown). DAB: 3,3′-diaminobenzidine. Bar = 250 μm.
