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. 2017 Mar 30;7:45407. doi: 10.1038/srep45407

Figure 1. Effect of ATP removal on TASK-2-mediated currents.

Figure 1

K+ currents recorded at 0 mV in HEK-293 cells expressing TASK-2 immediately after breaking into whole-cell recording (0 min) or after 20 min in cells dialysed with 1 mM ATP (A) or with the nucleotide omitted from the intracellular solution (B). The holding potential was −70 mV and the pulse was to 0 mV. (C and D) Corresponding current-voltage relations obtained from voltage ramps. (E) Time course of the K+ currents (means ± SEM) recorded at 0 mV in HEK-293 cells expressing TASK-2 after gaining access into the cells with pipette solutions containing 1 mM ATP (n = 10), no ATP (n = 9) or ATP-γ-S (n = 4). (F) Recording from an excised inside-out patch of membrane taken from a HEK-293 cell over-expressing TASK-2. The patch was held at −60 mV and at the indicated times intracellular 1 mM ATP was removed from the intracellular solution. During the time indicated by the black bar intracellular pH was decreased from 7.4 to 5.5. (G) Average currents measured before and after ATP removal (means ± SEM, n = 21). The observed decrease in current was statistically significant when measurements were compared with a paired test (**P < 0.001).