Figure 3. Gq protein coupled receptor inhibition of TASK-2 and minor effect of diacylglycerol.

(A) Effect of activation of the angiotensin II receptor AT₁ on TASK-2-mediated currents. HEK-293 cells were co-transfected with AT₁ and TASK-2, or the TASK-2-K257A-K258A mutant. Current was monitored by brief pulses to 0 mV from a holding potential of −75 mV. Angiotensin II at a concentration of 1 μM was added during the indicated time. Results are means ± SEM of 6 experiments for TASK-2 and 4 experiments for TASK-2-K257A-K258A. (B) Effect of a diacylglycerol analogue assayed on TASK-2- and TASK-3-expressing cells. Current was monitored by brief pulses to 0 mV from a holding potential of −75 mV. The box labelled DAG indicates the addition of diacylglycerol short-chain analogue 1,2-dioctanoyl-sn-glycerol at 100 nM. Results are means ± SEM of 5 experiments for TASK-2 and 8 experiments for TASK-3.