Table 1. Functional effect of cysteine mutations in certain TASK-2 positively-charged residues.
| Mutation | I at 80 mV | MTSET effect | VSP inhibition | Neomycin inhibition |
|---|---|---|---|---|
| R4C | ✓ | — | — | undet |
| K254C | ✓ | ✓ | — | undet |
| K297C | ✓ | ✓ | ✓ | undet |
| R438C | — | — | ✓ | ✓ |
✓ under “I at 80 mV” indicates a significantly decreased whole cell-current with respect to WT TASK-2. ✓ under “MTSET effect” indicates a significant increase in current after treatment with the methanesulfonate reagent. ✓ under “VSP inhibition” reveals an accelerated inhibition of the mutant compared with the speed of inhibition of WT TASK-2 by activation of cotransfected DrVSP. ✓ under Neomycin inhibition indicates a significantly lower IC50 for inhibition by neomycin compared to control. — in all cases stands for no significant effect. Undet, undetermined.