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. 2017 Mar 30;7:45367. doi: 10.1038/srep45367

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Depiction of the three major glycosphingolipid series - globo, ganglio, and (neo-) lacto series glycosphingolipids. B3GNT5 attaches GlcNAc to the LacCer synthesizing Lc3, the precursor of all nsGSLs. Glycosidic linkages are displayed next to CFG notated monosaccharides. (B) Representative histograms of flow cytometry data on normal and cancer cell lines stained for paragloboside (nLc4, green) and P₁ (red). Grey histogram depicts the negative control. Values within each plot show the mean out of three independent experiments for nLc4 (green) and P₁ (red).