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. 2017 Mar 30;7:45367. doi: 10.1038/srep45367

Figure 4. Loss of gangliosides by B4GALNT1-editing does not affect α2–6 sialylation in IGROV1 cells.

Figure 4

(A) Depiction of CRISPR-Cas9-mediated B4GALNT1 editing in IGROV1 cells using two different sgRNAs [red; PAM sequence (green)]. In silico analysis revealed a deletion of 917 bp including translation start site located at exon 2. (B) Verification of ∆B4GALNT1 cells by DNA sequencing. (C) Representative flow cytometry zebra blot for parental IGROV1 and ∆B4GALNT1 cells. (D) Bar chart summarizing three independent flow cytometry experiments. (E) SNA-staining remains unaffected in ∆B4GALNT1 cells compared to IGROV1 (B4GALNT1). Data are represented as mean ± s.d.