Table 1.
Method validation parameters including accuracy and precision determined at two different concentrations levels and recovery with standard deviation (STD, n = 3).
| Compounds a | Accuracy (RE %) | Intraday (RSD %) | Interday (RSD %) | Recovery (%) | R2 | Linearity Range (µg∙g−1) | MDL (µg∙g−1) | MQL (µg∙g−1) | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| L | H | L | H | L | H | L | H | ||||||
| 1 | MMPB b,c | 8 | 6 | 7 | 9 | 12 | 12 | 79 (9) | 85 (10) | 0.9988 | 0.6–20 | 0.2 | 0.6 |
| ANA-a b | 6 | 5 | 6 | 7 | 11 | 13 | 95 (7) | 92 (8) | 0.9987 | 0.8–20 | 0.3 | 0.8 | |
| 2 | ANA-a | 9 | 7 | 5 | 5 | 8 | 9 | 89 (7) | 90 (8) | 0.9995 | 0.1–20 | 0.04 | 0.1 |
| MC-RR | 7 | 8 | 7 | 3 | 11 | 8 | 86 (9) | 89 (8) | 0.9993 | 0.03–20 | 0.01 | 0.03 | |
| MC-YR | 8 | 4 | 5 | 2 | 12 | 9 | 85 (7) | 90 (6) | 0.9992 | 0.07–20 | 0.02 | 0.07 | |
| MC-LR | 6 | 3 | 7 | 6 | 9 | 7 | 91 (10) | 95 (10) | 0.9995 | 0.06–20 | 0.02 | 0.06 | |
| MC-LA | 6 | 4 | 5 | 1 | 9 | 11 | 92 (7) | 91 (9) | 0.9990 | 0.03–20 | 0.01 | 0.03 | |
| MC-LY | 7 | 6 | 8 | 5 | 7 | 9 | 87 (8) | 92 (8) | 0.9995 | 0.1–20 | 0.03 | 0.1 | |
| MC-LW | 10 | 7 | 9 | 4 | 9 | 10 | 96 (7) | 94 (9) | 0.9993 | 0.05–20 | 0.02 | 0.05 | |
| MC-LF | 8 | 5 | 10 | 2 | 11 | 8 | 93 (9) | 90 (7) | 0.9992 | 0.1–20 | 0.03 | 0.1 | |
| 3 | CYN | 11 | 10 | 11 | 9 | 15 | 12 | 88 (10) | 85 (7) | 0.9990 | 0.1–20 | 0.04 | 0.1 |
| STX | 12 | 10 | 12 | 8 | 13 | 13 | 83 (11) | 86 (9) | 0.9989 | 0.3–20 | 0.1 | 0.3 | |
| BMAA | 8 | 7 | 8 | 8 | 10 | 12 | 97 (6) | 94 (5) | 0.9993 | 0.08–20 | 0.02 | 0.08 | |
a Validation results are divided between the three methods. L (low concentration) and H (high concentrations) are respectively set as the follow: 1 and 10 µg∙g−1 (1), 0.1 and 10 µg∙g−1 (2), and 0.5 and 10 µg∙g−1 (3). b Analysis using LDTD-APCI-HRMS detection. c Results reported as total MCs equivalent.